Science

A new biotinylation enzyme for analyzing protein-protein interactions

A new biotinylation enzyme for analyzing protein-protein interactions

Proteins play roles by interacting with various other proteins. Therefore, interaction analysis is an indispensable technique for studying the function of proteins. In this research, we have developed a biotinylation enzyme, AirID, using an ancestral enzyme reconstruction algorithm. AirID is a highly active biotinylation enzyme with low toxicity. By using AirID, comprehensive biotinylation of proteins interacting with a target protein in cells was achieved when the target protein was expressed as a fusion protein with AirID.

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